Composite

Part:BBa_K3939666:Design

Designed by: Yu Huang   Group: iGEM21_Tianjin   (2021-09-30)


Gal Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 708
    Illegal BglII site found at 1645
    Illegal BamHI site found at 2463
    Illegal XhoI site found at 4001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3197
    Illegal AgeI site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When the inducer is galactose, the regulation effect may not be good because galactose can be used as carbon source. In addition, regulation of sequence expression by Gal may result in sequence expression leakage.


Source

From the lab.

References